Figure 3
Identification and validation of CSDR-miRNA targets. (a) Molecular function enrichment analysis showing the enrichment of “GO:005507-Copper-ion binding” among the CSDR-miRNA targeted 402 genes. Targets were identified through degradome as well as literature. (b) CSDR-miRNA:target interaction map showing the association of most valid targets with GO:005507 (Copper ion binding) term. The target node size corresponds to degradome read number (smallest node ≤ 10 reads and biggest node with ≥200 reads). (c) Expression analysis of genes targeted by miR528-5p, miR398b, miR1425-5p, miR1871 and miR435. For each real-time expression analysis, at least three biological replicates with three technical replicates were analyzed here. The error bars represent the standard error. Actin was used as the endogenous control.
