Table 1 List of antibodies used for multiparametric NKT cells analyses in this study.
From: NF-κB Protects NKT Cells from Tumor Necrosis Factor Receptor 1-induced Death
Antigen | Fluorochrome* | Clone | Source** |
|---|---|---|---|
CD1d-tetramer | APC, BV421 | na | in-house73; NIH Tetrmer Core (Emory University) |
CD3ε | PE, PerCP-Cy5.5, APC, PE-Cy7 | 145-2C11 | BD Biosciences, BioLegend, Tonbo Biosciences |
CD8α | FITC, PerCP-Cy5.5, APC-Cy7 | 53–6.7 | BD Biosciences, BioLegend, eBiosciences |
CD44 | FITC | IM7 | BD Biosciences |
NK1.1 | PE, PerCP-Cy5.5, APC, APC-Cy7 | PK136 | BD Biosciences |
B220 | FITC, PerCP-Cy5.5, APC-Cy7 | RA3-6B2 | BD Biosciences |
CD4 | FITC, PE, PerCP-Cy5.5 | RM4-4 | BD Biosciences, BioLegend |
CD69 | FITC, PE, PE-Cy7 | H1.2F3 | BD Biosciences |
CD122 | PE | TM-b1 | BioLegend |
Ly6C | FITC, APC-Cy7 | AL-21 | BD Biosciences |
Ly49C/I | FITC | 5E6 | BD Biosciences |
CD62L | APC-Cy7 | MEL-14 | BD Biosciences |
CD25 | PerCP-Cy5.5, PE-Cy7 | PC61 | BD Biosciences |
TNFR1 | PE | 55R-286 | BioLegend |
TNFR2 | PE | TR75-89 | BioLegend |
Isotype control | PE | HTK888 | BioLegend |
OX40 | PE | OX-86 | BioLegend |
Isotype control | PE | RTK2071 | BioLegend |
GITR | PE | DTA-1 | BioLegend |
Isotype control | PE | RTK4530 | BioLegend |
CD95/Fas | PE | Ja2 | BD Biosciences |
Isotype control | PE | Ha4/8 | BD Biosciences |
