Figure 5

Quantitative RT-PCR validation of expression of terpene synthase genes selected from the DGE analysis in S. officinalis. Total RNAs were extracted from young leaves, old leaves, stem, flower and bud flower samples and the expression of SoNEOD, SoGPS, SoFPPS, SoGGPS, SoMYRS, SoLINS, SoHUMS, SoTPS6, SoSQUS, SoSABS and SoCINS genes were analysed using quantitative real-time. SoACTIN was used as the internal reference. The values are means ± SE of three biological replicates.