Figure 1
From: Acinar injury and early cytokine response in human acute biliary pancreatitis
Structural and functional integrity of acini prior to induction experiments. (a) Inverted microscopic image of acinar clusters with apically located zymogen (white arrows). (b) H&E image showing an acinus with basally located nuclei (white arrows). (c) Transmission electron microscopic image showing part of an acinus with a polarized acinar cells showing basally located nuclei and apically located zymogen (yellow arrow). (d) IF image showing amylase stain within acinar cells distributed predominantly in the apical region (white arrows). (e) Line diagram representing dose dependent amylase secretion in response to stimulation with incremental doses of carbachol. Maximal amylase secretion was observed with 10 μM of carbachol, which was significantly higher compared to controls (*indicates p < 0.05). Thereafter, there was persistent reduction of amylase secretion with higher doses of carbachol. (f) Histograms showing the effect of atropine on amylase secretory response after stimulation of pancreatic acini with 10 μM carbachol. Pre-treatment of the acini with 10 μM atropine for 20 mins resulted in reduction in amylase secretion. The error bars indicate standard error of mean (SEM). The figures represent data from at least 3 independent experiments.
