Figure 1

Chemical screening strategy and the effect of cabergoline. (a) RT-PCR analysis of steroidogenic enzyme mRNA expression in OSR1⁺ cells. (b) Schematic representation of induction of undifferentiated induced pluripotent stem cells into intermediate mesoderm and screening strategy used. (c) Effect of cabergoline on OSR1⁺ cells treated with 1 μM cabergoline for 4 days and then stained with an anti-3β-HSD antibody and DAPI. Scale bar, 50 μm. (d) Dose-dependent 3β-HSD2 mRNA expression curve in cabergoline-treated OSR1⁺ cells. Levels of 3β-HSD2 mRNA expression are shown as relative values compared with those of DMSO-treated controls. Expression levels are normalised to that of a housekeeping gene, β-actin. Student’s t-test was used for comparison between the experimental and control group. *P < 0.05. Data represent mean ± SEM of four independent experiments (n = 4).