Table 1 Levels of steroid hormones secreted into culture medium.

From: Significance of dopamine D1 receptor signalling for steroidogenic differentiation of human induced pluripotent stem cells

 

Control

ACTH

ACTH + SKF 83822

Cortisol (pg/ml)

415 ± 19.9

**560 ± 26.2

* †1136 ± 215

Aldosterone (pg/ml)

14.5 ± 0.9

19.5 ± 2.0

*27.1 ± 3.5

DHEA (pg/ml)

2139 ± 278

2291 ± 274

*3059 ± 126

  1. Culture medium was collected for steroid hormone measurements after a 48 h of incubation with 2.4 µM ACTH and/or 1 µM SKF 83822. Statistical analysis was performed using two-tailed unpaired Student’s t-test with Holm-Bonferroni correction. *, vs Control P < 0.05; **, vs Contol P < 0.01; †, vs ACTH P < 0.05. Data represent mean ± SEM of five independent experiments (n = 5).
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