Figure 2 | Scientific Reports

Figure 2

From: Novel method for rapid fluorescence in-situ hybridization of ALK rearrangement using non-contact alternating current electric field mixing

Figure 2

Device used to apply a high voltage, high frequency AC electric field (4.5 kv, 90 Hz). The DNA probes are mixed within microdroplets as the voltage is switched on and off. The resultant coulomb force stirs the probe solution on the sections, which increases the opportunity for contact between the probe and gene. Note that although the microdroplets’ shape is not transformed, the solution is stirred in Supplementary Video 1. (A) The device for Rapid Fluorescence in Situ Hybridization (RaFISH). (B) Polyethylene terephthalate (PET) cover on the slide for inhibiting evaporation of the probe solution. (C) Schema of the stir within a microdroplet as the voltage is switched on and off.

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