Figure 5

Correlation between signaling protein phosphorylation and IFN-γ production by T cell subsets. (a) Gating strategy for the production of IFN-γ by CD4⁺CD28⁺ (left), CD8⁺CD28⁺ (middle) and CD8⁺CD28⁻ (right) cells, in both unstimulated and stimulated samples. (b) Correlations between p-p38MAPK (upper graphs), p-ERK (middle graphs) and p-Akt (lower graphs) and the production of IFN-γ by CD4⁺CD28⁺ (left), CD8⁺CD28⁺ (middle) and CD8⁺CD28⁻ (right) cells after stimulation with PMA/ionomycin. Correlations were calculated before transplantation and 90 days after transplantation. P-p38MAPK significantly correlated with the production of IFN-γ in CD8⁺CD28⁺cells at day 90 after transplantation. (n = 12 before transplantation and n = 15 90 days after transplantation); *p < 0.05, **p < 0.01, ***p < 0.001.