Figure 9

(A) Brightfield image of cellular shedding from LPA-treated SKOV3ip has (B) corresponding CARS image of −2930 cm⁻¹ signal. (C) The maximum intensity CARS spectrum and average spectrum of the map show similar chemical composition as non-treated SKOV3ip samples. (D) The representative CARS spectrum and nonlinear peak fit results provide the resonant CH₂ and CH₃ components used for (E) the frequency scatter plot separating the chemical contributions of lipids (red) and proteins (blue). (F) The reconstructed map shows the chemical distribution of the segmented lipid (red) and protein (blue) pixels. Experimental parameters: Pump = 720 nm, 25.20 mW; Supercontinuum = 785–945 nm, 3.80 mW; Acquisition time = 500 ms/pixel; Map size = 51 × 51 pixels; Steps = 300 nm; Objective = 100x, 0.9 NA.