Table 1 Primers used for qRT-PCR gene expression analysis.

From: MiR-17-5p and miR-20a promote chicken cell proliferation at least in part by upregulation of c-Myc via MAP3K2 targeting

Gene name

Forward/reverse primers (5′-3′)

Ki67

F: AGGATGGAAGCAAGTCACCTGGAT R: CTTCTGAACGGGGACTGGAATCTT

PCNA

F: CGTCTCATGTCTCCTTGGTGCA R: GGACATGCTGGTGAGGTTCA

Cyclin D1

F: CTCGGAGCTACCTGCATGTTTGT R: TTTACGGATGATCTGTTTGGTGT

E2F1

F: CCCCGCACCTCCTCATCGTCT R: CAAGTTCAGCTTCCGCTTCACCG

NONO

F: AGAAGCAGCAGCAAGAAC R: TCCTCCATCCTCCTCAGT

Pri-miR-17-92

F: CATCTACTGCCCTAAGTGCTCCTT R: GCTTGGCTTGAATTATTGGATGA

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