Figure 4 | Scientific Reports

Figure 4

From: Blastocyst Formation Rate and Transgene Expression are Associated with Gene Insertion into Safe and Non-Safe Harbors in the Cattle Genome

Figure 4

Representative FACS analysis of different transfected clones for expression of EGFP. (A) Three transgenic clones contained CpG-free EF1α cassette integrated at BF4, BF10 and BF5 respectively. (B) Three transgenic clones contained CMV cassette integrated at BF4, BF10 and BF5 respectively. (C) Three transgenic clones contained EF1α cassette integrated at BF4, BF10 and BF5 respectively. Un-transfected fibroblasts were used as a negative control.

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