Figure 4

(a) aAPs were treated with or without HPI-4 for 24 h. Immediately after (0 h), 24 h or 48 h after HPI-4 removal cells were fixed and Ac-Tub (in green) was revealed by immunocytochemistry. (b) Cells treated as described in A were treated for three days with TGF-β1. α-SMA (in red) was revealed by immunocytochemistry. The white bar represents 20 μm.