Figure 5

aAP were treated for 3 days with TGF-β1 then with or without HPI-4 for 24 h. HPI-4 was removed and, after 3 days, (a) cells were fixed and α-SMA was revealed by immunocytochemistry and (b) RNA were extracted and α-SMA and COL1A1 expression was assessed using quantitative RT-PCR (mean + /− sd of a representative experiment (*p < 0.05 **p < 0.01 n = 3).