Table 1 Growth times of human Sertoli cells cultured in vitro.
Donor age (years) | Thawing of cells | Growth medium | Time needed to reach ~70–80% confluence (days) | |||||
|---|---|---|---|---|---|---|---|---|
P1 | P2 | P3 | P4 | P5 | P6 | |||
36 | Time 0 | F12/DMEM + 10% FBS + S/P (1:100)* | 21 | 8 | 13 | |||
40 | 10 | 5 | 5 | 7 | 5 | 8 | ||
15 | 22 | 12 | 10 | 12 | ||||
23 | 23 | 19 | 11 | |||||
