Figure 4
From: Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity
Crystal structures of GFP-clamps, as well as the individual DARPins 3G61 and 3G124nc, in complex with eGFP. (a) Complex between 3G124nc and eGFP; Complex is shown as found in the crystal structure on the left and in “open book” view on the right to show the binding interface. (b) Same representation as in (a) for the complex between a GFP-clamp (gc_K7) and eGFP. (c) Same representation as in (a) for the complex between 3G61 and eGFP. Interacting atoms in (a–c) are coloured according to their distance from the binding partner (red:<3.6 Å, orange: <5 Å, yellow: whole residues with some atoms closer than 5 Å). (d) Superposition of the parental DARPins and a representative experimental structure of a GFP-clamp (gc_K11).
