Figure 5 | Scientific Reports

Figure 5

From: Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity

Figure 5The alternative text for this image may have been generated using AI.

Protein purification using GFP-clamps. A fusion protein between sfGFP and maltose binding protein (GFP_MBP) is purified from crude E. coli extract with a GFP affinity resin. M: Protein size marker; CE: crude extract; FT: flow-through, E: elution with 3C-protease; RI: purified MBP after removal of His-tagged 3C-protease by reverse IMAC. The gel image was recorded from a single gel.

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