Table 3 Strains identified by SID and delta amplification in must samples.

From: SaccharomycesIDentifier, SID: strain-level analysis of Saccharomyces cerevisiae populations by using microsatellite meta-patterns

Sample

Must type

Inoculum

SID strains

GLMerror1

delta amplif. strains

cluster2

Pied de cuve

Muller-Thurgau

Pied de cuve

02_MF; 03_MF

3.08*

A

A = 02_MF/03_MF

B

B = 02_MF/03_MF

C

C = 02_MF/03_MF

MullerMust

Muller-Thurgau

none

02_MF; 03_MF

2.16

B

B = 02_MF/03_MF

C

C = 02_MF/03_MF

Muller_Pdc_4dd

Muller-Thurgau

Pied de cuve

02_MF; 03_MF

2.16

A

A = 02_MF/03_MF

Muller_Pdc_8dd

Muller-Thurgau

Pied de cuve

02_MF; 03_MF

1.54

A

A = 02_MF/03_MF

B

B = 02_MF/03_MF

Muller_Pdc_EF

Muller-Thurgau

Pied de cuve

02_MF; 03_MF

2.16

A

A = 02_MF/03_MF

B

B = 02_MF/03_MF

Muller_prep1_4dd

Muller-Thurgau

Starter blend1

BR120; M57_num652;prep1_isolate

4.97

D

D = M57_num652

Muller_prep1_8dd

Muller-Thurgau

Starter blend1

BR120; M57_num652;prep1_isolate

4.97

C

C = 02_MF/03_MF

D

D = M57_num652

Muller_prep1_EF

Muller-Thurgau

Starter blend1

BR120; M57_num652;prep1_isolate

4.97

D

D = M57_num652

SauvignonMust

Sauvignon

none

02_MF; 03_MF

2.16

C

C = 02_MF/03_MF

Sauvignon_Pdc_4dd

Sauvignon

Pied de cuve

02_MF; 03_MF

1.54

A

A = 02_MF/03_MF

Sauvignon_Pdc_EF

Sauvignon

Pied de cuve

02_MF; 03_MF

2.16

A

A = 02_MF/03_MF

B

B = 02_MF/03_MF

Sauvignon_prep1_4dd

Sauvignon

Starter prep1

BR120; M57_num652;prep1_isolate

4.97

D

D = M57_num652

Sauvignon_prep1_EF

Sauvignon

Starter prep1

BR120; M57_num652;prep1_isolate

4.97*

D

D = M57_num652

F

F = M57_num652

Sauvignon_prep2_4dd

Sauvignon

Starter prep2

prep2_isolate

5.57

G

G = prep2_isolate

Sauvignon_prep2_EF

Sauvignon

Starter prep2

prep2_isolate

4.02*

G

G = prep2_isolate

E

G = prep2_isolate

ChardonnayMust

Chardonnay

none

02_MF; 03_MF

2.16

A

A = 02_MF/03_MF

B

B = 02_MF/03_MF

C

C = 02_MF/03_MF

SolarisMust

Solaris

none

02_MF; 03_MF

2.16

A

A = 02_MF/03_MF

B

B = 02_MF/03_MF

TraminerMust

Traminer

none

02_MF; 03_MF

2.16

A

A = 02_MF/03_MF

B

B = 02_MF/03_MF

  1. 14dd, 8dd, EF = samples were collected 4 and 8 days after the inoculum and at the End of Fermentation, respectively. The strains present in the samples were identified by means of microsatellites amplification on the total extracted DNA followed by analysis with SID (SID strains). In addition, strains were isolated from samples and characterized by means of delta amplification, and the band patterns were compared to assess the number and type of strains present (delta amplif. strains). 1GLMerror was calculated as the percentage of alleles differing between the predicted profile and the query sample on the total number of alleles in the predicted profile (equation 3 in materials and methods). All the predicted profiles were composed by a total of 324 alleles, with the exception of these annotated with *, which encompassed 325 alleles. 2According to the microsatellites profiles, as shown in Supplementary Figure S4.
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