Figure 2
Sensitivity of PNS and photostability studies. (a) Dose-dependent response of PNS to H2O2 in solution. PNS was exposed to H2O2 in pH 7.5 phosphate buffered saline at 37 °C for 45 min. Integrated fluorescence intensity is shown at 510 nm–520 nm (carboxy-PF1, shown in green; excitation 450 nm) and at 690 nm–710 nm (NV nanodiamond, shown in red; excitation 550 nm). Each data point was collected in triplicate and averaged, and error bars show the standard deviation at each concentration. (b) Photobleaching of PF1 (green, excitation 485 nm, emission 520 nm), RPF1 (blue, excitation 400 nm, emission 520 nm) and NV nanodiamonds (red, excitation 560 nm, emission 700 nm). Excitation intensity was 12 Wcm−2, emission was normalised for each sample at t = 0 s and an image was recorded every 0.5 s.
