Figure 4
Long-term imaging of PNS by confocal microscopy. Images of a representative macrophage cell after 13.5 h showing (a) red emission (excitation 560 nm, emission 680–720 nm) of NV nanodiamond, (b) green emission (excitation 476 nm, emission 510–520 nm) of surface bound carboxy-PF1, and (c) brightfield contrast of the same cell. (d) Integrated fluorescence of the NV nanodiamond emission of PNS over 13.5 h of confocal microscopy (63x magnification, averaged results from three locations). (e) Fluorescence ratio of carboxy-PF1 to NV nanodiamond, which increased by 18% (p = 0.032) over the course of the 13.5 h experiment. All error bars are the standard error of the mean.
