Figure 3
From: The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus
Subcellular localization of M25 proteins during the MCMV infection cycle. (a,b) MEF infected with the vM25HA virus for the indicated time periods were analyzed by confocal microscopy after labeling with antibodies specific for the HA epitope, lamin B and the Golgi protein GM130. Size bar, 10 µm. (c) NIH 3T3 cells were infected with the vM25HA virus. At the indicated time points either total cell lysates (T) or cytoplasmic (C) and nuclear (N) fractions were prepared and analyzed by immunoblotting with the M25-specific antibody. Fractionation was verified for lamin B and β-tubulin.
