Figure 2
From: Systems pathology by multiplexed immunohistochemistry and whole-slide digital image analysis
Multiplexed immunohistochemistry for epithelial cells in prostate tumour. (a) Fluorescence (FL) and brightfield (CHR) images were acquired and (b) images are registered using nuclei information from both images, namely Hoechst and haematoxylin. (c) Cell segmentation and classification is based on epithelium marker expression (Pan-Epi = Pan-CK + E-cadherin) and cell-type specific classifier marker expression in each prostate gland. A given gland is classified as cancerous if basal cell marker expression (CK5 + p63) is lost. Scale bar 500 µm. AMACR, alpha-methylacyl-CoA racemase; AR, androgen receptor; CK5, cytokeratin 5; CK8, cytokeratin 8; CK18, cytokeratin 18; CHR, chromogenic; FL, fluorescence; IHC, immunohistochemistry; Pan-CK, pan-cytokeratin; Pan-Epi; pan-epithelium.
