Figure 1 | Scientific Reports

Figure 1

From: Mild heat induces a distinct “eustress” response in Chinese Hamster Ovary cells but does not induce heat shock protein synthesis

Figure 1

The effect of temperature on HSP induction and HSF1 activation. (A) A GPI-mGFP–expressing CHO cell line was incubated for 20 min at the specified temperatures; the samples were prepared for western blotting after 6 h of recovery at 37 °C. (B) HSF1 phosphorylation was analyzed in samples after 20 min of heat treatment without recovery. See Supplementary Figure S5 and S6 for the full images of Fig. 1A,B, respectively.

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