Figure 5 | Scientific Reports

Figure 5

From: Mild heat induces a distinct “eustress” response in Chinese Hamster Ovary cells but does not induce heat shock protein synthesis

Figure 5

The alteration of membrane diffusion of GPI-mGFP in live cells at different heat exposures. The diffusion coefficient (D) and confinement time (τ 0) of GPI-mGFP on the surface of CHO cells were measured by ImFCS (A) during a 15 min primary treatment (1st heat) at 37 °C, 40 °C, and 42.5 °C; (B) after a 6 h recovery at 37 °C (37 °C), and upon a 15 min second heating cycle (2nd heat) at the same temperature as in (A). (C) ImFCS analysis of PM sheets isolated from CHO cells, at 37 °C, 40 °C, or 42.5 °C. The values represent averages, and the error bars represent the standard error of mean (SEM) (n = 8, p < 0.05).

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