Figure 6 | Scientific Reports

Figure 6

From: Mild heat induces a distinct “eustress” response in Chinese Hamster Ovary cells but does not induce heat shock protein synthesis

Figure 6

Characterization of lipidomes of GPI-mGFP–expressing CHO cells exposed to different levels of HS. (A) sPLS-DA and SoamD score (inset) of lipid molecular species. (B) Heatmap representation of hierarchical clustering of lipid profiles at 37 °C, 40 °C, 42.5 °C, and 44 °C. The top 30 most significant species were selected using ANOVA, Euclidean distance, and clustering algorithm Ward. The heat color-code represents normalized values (z-scores), ranging between [−2] (darkest shade of blue) and [+2] (darkest shade of red). Data from four independent experiments with control and HS-treated cells are shown. (C) Key features of HS-associated lipid remodeling of CHO lipidomes: ceramide (Cer), phosphatidylserine (PS), phosphatidylglycerol (PG), cardiolipin (CL), lysophosphatidylcholine (LPC), and phosphatidic acid (PA). The cells were either unstressed (37 °C) or stressed at 40 °C, 42.5 °C, or 44 °C, for 20 min. The values are expressed as mol% of membrane lipids (mean +SD), n = 4; *p < 0.05, (vs. 37 °C).

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