Figure 5

HGF regulates target genes of miR-7. The expression of selected target genes for miR-7 was examined after treatment of the MCF-10A cells with HGF protein (A) and siRNA (B) to induce upregulation or downregulation of HGF, respectively. Twenty-four hours after treatment, the expression level of target genes was measured by qPCR. All the target genes were downregulated by HGF but upregulated by siRNA against HGF, except for SET8. All experiments were performed in triplicates and indicated as mean ± SE. *P < 0.05; **P < 0.001.