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Figure 1

From: 5-Aminolevulinic Acid Guided Sampling of Glioblastoma Microenvironments Identifies Pro-Survival Signaling at Infiltrative Margins

Figure 1

Study workflow. (a) T1-weighted MRI stealth scan showing a contrast-enhancing GBM with localization during surgery. (b) Fluorescent view of surgical field during resection. White arrow points to fluorescent tumor bulk tissue after accumulation of protoporphyrin IX (PpIX) and excitation at λ = 400–410 nm using ultraviolet blue light. (c) Study workflow. Tumor microenvironments were distinguished by the presence or absence of PpIX fluorescence due to 5-ALA administration. Protein was isolated from PN, BT, and TM tissue samples and Reverse Phase Protein Array (RPPA) was performed. Data was then processed and oncogenic proteins were chosen for validation studies and immunohistochemistry (IHC).

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