Figure 4 | Scientific Reports

Figure 4

From: 5-Aminolevulinic Acid Guided Sampling of Glioblastoma Microenvironments Identifies Pro-Survival Signaling at Infiltrative Margins

Figure 4

Validation of protein expression using TCGA data, immunohistochemistry, and western blotting. (a) Selected box plots of normalized protein expression intensity values for each tumor region (TM, BT, PN) in our dataset (b) and in the TCGA dataset (GBM & IDH-wt grade II/III glioma) displaying differential protein expression patterns conserved in both datasets. Asterisk indicates significant difference (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). TM n = 22, BT n = 18, PN n = 14, IDH-wt grade II/III glioma n = 76, GBM n = 136. (c) Western blotting for Akt (pT308), Akt (pS473), Fibronectin, Bcl-XL, and PAI-1 using corresponding patient tissue samples. n = 3 patients for each tumor region. Proteins were probed for on two separate blots. Uncropped full length blots can be found in Supplemental Fig. 4. (d) Immunohistochemical validation of selected proteins using formalin-fixed, paraffin-embedded GBM tissue samples corresponding to those used in RPPA. Images were acquired at 60x magnification.

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