Figure 3
From: Chemokines protect vascular smooth muscle cells from cell death induced by cyclic mechanical stretch
Induction of CXCL1 and CX3CL1 in RASMCs subjected to CMS. RASMCs were incubated with SP600125 (20 μM) for 20 min and then subjected to CMS for four hours. Cells were harvested and analyzed by real-time RT-PCR with specific primers for Cxcl1 (a) or Cx3cl1 (b) or by immunoblotting using the anti-CX3CL1 antibody (e), and media were analyzed using ELISA for CXCL1 (c) or CX3CL1 (d). (f) The quantity of CX3CL1 expressed in RASMCs, as measured by scanning densitometry, is expressed as a percentage of the control, normalized with respect to beta-actin. Data are means ± SE (n = 3), and N.S. indicates no significant difference. RASMCs were incubated with BAY 11-7082 (5 µM) for 20 min and then subjected to CMS for four hours. Cells were harvested and analyzed by real-time RT-PCR with specific primers for Cxcl1 (g) or Cx3cl1 (h). The uncropped pictures of immuoblotting are shown in Supplemental Fig. S4 (a,b).
