Figure 3
From: Andy’s Algorithms: new automated digital image analysis pipelines for FIJI
A new pipeline for image quantification for proximity ligation assays. (A) Flow chart depicting the image processing steps within the PLA particle analysis algorithm for the selection of all positive PLA foci. (B) Representative raw PLA image (top left panel) and the selection overlays for nucleus (top right, red), cytoplasm (bottom left, blue) and PLA foci (bottom right, green). (C) Scatter plot depicting the number of PLA positive foci within the nucleus, cytoplasm or whole cell (total) and compared to control calculated with Andy’s PLA algorithm and compared data from37 quantified using Imaris software (n = 7 per group, ANOVA p-value tests variance across algorithms).
