Figure 4
Cellular metabolism after exposure to s-microgravity. (a–c) Intracellular Ca2+, ROS and O2 •− levels, respectively, in live cells grown at 1 g (Ctr) or exposed to s-microgravity (RPM); values are expressed as the ratio between fluorescence emission (f) and the number of loaded cells at each time point. Values are presented as means ± SEM, n = 5. ***p < 0.001 compared with the corresponding Ctr. (d) Mitochondrial membrane potential in live cells grown under the same conditions described in (a–c); values are expressed as the ratio between red and green fluorescence at each time point. Values are presented as means ± SEM, n = 8. ***p < 0.001 compared with the corresponding Ctr.
