Figure 2 | Scientific Reports

Figure 2

From: Identification of host DEAD-box RNA helicases that regulate cellular tropism of oncolytic Myxoma virus in human cancer cells

Figure 2

Effects of selected RNA helicase siRNAs on early and late gene expression of MYXV in non-permissive PANC-1 cells. PANC-1 cells were plated in 48 well plates, transfected with control non-targeting siRNA (siCon) or siRNAs targeted to the indicated genes. After 48 h, the cells were infected with vMyx-GFP-TdTomato at MOI of 1 FFU/cell and virus-containing media was replaced with fresh media after 1 h of virus adsorption. Images showing GFP (top panels) and TdTomato (bottom panels) of the siRNA-transfected and virus-infected PANC-1 cells were taken 72 h after infection using a Leica fluorescence microscope.

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