Figure 1 | Scientific Reports

Figure 1

From: HDL particles incorporate into lipid bilayers – a combined AFM and single molecule fluorescence microscopy study

Figure 1

GUVs made of DOPC were incubated with fluorescently labelled HDL. (A) Representative images of a single GUV in the C-Bodipy channel (green) and the apoA-I-Alexa647 channel (red) are shown (left). The right panel shows the C-Bodipy versus the apoA-I-Alexa647 signal for single vesicles. (B,C) FCS and FCCS on apoA-I-Alexa647 and C-Bodipy. Shown are FCS and FCCS curves for the indicated probe molecules after fusion of HDL particles with GUV membranes (B) or in buffer solution (C). Panel (C) shows high cross-correlation when HDL particles were intact (red and pink curves virtually identical) whereas panel (B) shows no notable cross-correlation after HDL fusion with the DOPC membrane. Black solid lines represent the fits.

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