Figure 2 | Scientific Reports

Figure 2

From: HDL particles incorporate into lipid bilayers – a combined AFM and single molecule fluorescence microscopy study

Figure 2

HDL incorporates into supported lipid bilayers. (A) High-speed AFM images of native HDL on mica (left) and on a supported DOPC bilayer (right image). (B) Diffusion analysis of added HDL particles on supported DOPC bilayers. The plot shows the mean-square displacement versus time for inserted HDL particles (20 particles, frame rate = 12–20 frames per second). Diffusion was analysed with Eq. 2, yielding a diffusion constant of D = 10.44 ± 1.2 nm2/s. Error bars indicate the standard error of the mean. (C) Height analysis of native HDL particles (red) and reconstituted HDL particles (blue) adsorbed to mica (dashed lines) or DOPC bilayers (full lines). The histograms reveal two distinct peaks: the peak at 7.4 nm corresponds to the size of HDL particles, the peak at 4.0 nm to the height of particles incorporated into the lipid bilayer.

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