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Figure 1

From: Statistically strong label-free quantitative identification of native fluorophores in a biological sample

Figure 1The alternative text for this image may have been generated using AI.

(a–d) The artificial abundance map \({{\rm{s}}}_{[4\times 600]}\) of the total of 600 (24 × 25) pixels containing the fractions of each of four fluorophores (a) free NADH, (b) bound NADH, (c) porphyrin and (d) Cyt. c. This abundance map corresponds to case D. (e) The normalized reference spectra are shown for free NADH (blue), bound NADH (magenta), Cyt. c (cyan) and porphyrin (red). (f–w) Simulated hyperspectral image set (\({\boldsymbol{y}}={\boldsymbol{Ms}}+{\boldsymbol{n}}\)) assuming zero noise (\({\boldsymbol{n}}=0\)) taken in \(L=18\) different spectral channels. The corresponding channel numbers are given in Supplementary Material, Section 5. Images (f–w) are rescaled for better visualization as a grey colour-map, where the minimum value is displayed as black, and the maximum value (varying at each channel) is displayed as white (details of the colour ranges are given in Supplementary Table 2).

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