Figure 5
From: Development and physiological effects of an artificial diet for Wolbachia-infected Aedes aegypti
Characterization of the effects of ADM on key traits in wMel-infected A. aegypti. Experiments were conducted on either F1 (P generation fed either whole blood - Mel_WB (red), or ADM diet - Mel_ADM (crimson)) or F2 mosquitoes (where the P generation was fed as above, and the F1 generation fed on WB). Some experiments also involved a comparison against WT (Wolbachia-uninfected, black) mosquitoes fed on WB. Median (dot) and interquartile range (whiskers) wMel density values (relative to rps17) in F1 (a) and F2 (b) Mel_WB and Mel_ADM mosquitoes. Each line represents a family of 3–15 adult female mosquitoes. Slightly higher density levels were associated with F1 Mel_WB families, and F2 Mel_ADM families. MT = Proportion of samples that tested positive for wMel. Data were compared by 2-way ANOVA. (c & d) Two independent longevity experiments conducted on F1 WT (Wolbachia-uninfected), Mel_WB and Mel_ADM mosquitoes. There was no decrease in longevity associated with feeding ADM. Data were compared using Mantel-Cox test. Prevalence (proportion infected) and viral load (number of viral copies) at 7 (e) and 14 (f) days after oral infection with ZIKV for F1 WT, Mel_WB and Mel_ADM mosquitoes. The latter two treatments showed similar levels of resistance to ZIKV infection. Data were compared using Fisher’s exact test, and Mann-Whitney U test. Different letter codes represent statistically significant differences between treatments.
