Figure 3

Explained variance at the among-region level using analysis of molecular variance (AMOVA) in four Ephemeroptera species showing wide ecological distributions. Analyses were based on restriction-site-associated-DNA sequencing (RADseq) data. Probability of FRT (i.e., estimated variance among region/total estimated variance) was estimated with 1,000 permutations and P values were corrected for multiple-test bias following Bonferroni correction (alpha = 0.05/3 = 0.0167). Asterisks denote significant variance explained by the regional level, characterized by watersheds, volcanoes or elevation.