Table 1 Primer sequences and amplicon sizes for both the PCR and qPCR experiments.

From: Functional aspects of salivary nitric oxide synthase of Rhodnius prolixus (Hemiptera, Reduviidae) and nitric oxide trafficking at the vector-host interface

Target gene

Forward primer 5′-3′

Reverse primer: 5′-3′

Amplicon base pairs (bp)

NOSa

T7 + gtattcgattgccgtcaggt

T7 + gcaggacatcaaacatcgtg

332 bp

Mouse keratina

T7 + ggggtctcctctctggaaac

T7 + attagcagccgtggaagaga

275 bp

NP1a

T7 + tttgctgcagtgggtgtaag

T7 + agttgcccgacgttacatct

380 bp

NP2a

T7 + gccgtgaccattctctgtct

T7 + tcacggcgcttttaactttt

548 bp

NP3a

T7 + tggaaccgtactcagctttg

T7 + gcgtttttaaccgttgcact

566 bp

NP4a

T7 + cgtagccattctgtgcctgt

T7 + gcagatacggcgcttttaac

547 bp

α-tubulinb

tttcctcgatcactgcttcc

cggaaataactggggcataa

129 bp

NOSb

acgaagattggcgatactgg

gcgtacagagatggtgcaga

151 bp

NP2b

catatgtttgcgggaaggat

atcgtactggcacccaagat

163 bp

  1. aPCR primers (used for dsRNA template). bqPCR primers.