Figure 5 | Scientific Reports

Figure 5

From: Genome-wide survey indicates diverse physiological roles of the turnip (Brassica rapa var. rapa) calcium-dependent protein kinase genes

Figure 5

Interaction of BrrCDPKs with BrrRboh proteins. (A) Phylogenetic relationship of turnip Rbohs. A neighbor-joining tree was created for 15 turnip Rboh proteins using the MEGA7.0 program with 1000 bootstrap replicates. (B) Yeast two-hybrid analysis of interactions between CDPK and Rboh proteins in turnip. The yeast cells of strain AH109 containing the indicated plasmid combinations were grown on either the nonselective (SD-LW) or selective (SD-LWHA) media. AD is the empty pGADT7 vector. (C) Expression profiles of BrrRbohD1 and BrrRbohD2 under ACC, JA, SA, and pst DC3000 stress. Quantitative RT-PCR analyses were performed and expression values were calculated using the 2−△△CT method. Data are mean values ± standard error obtained from three replicates. Red indicates upregulated genes and green indicates downregulated genes. Asterisks denote statistically significant differences (t-test, p < 0.05).

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