Figure 6
H2O2 in guard cells and stomatal closure. (A) H2O2 content in leaves of turnip treated with pst DC3000 for 0, 1, and 3 h. Data are mean values ± standard error (SE) obtained from three replicates. Different letters within a column indicate a significant difference (p < 0.05; Tukey’s test). (B) DC3000-induced production of H2O2 by turnip leaf guard cells. Epidermal pieces of turnip leaves without (1 and 4) or with 1 h (2 and 5) or 3 h (3 and 6) of pst DC3000 treatment were loaded with 50 µmol/L H2DCF-DA for 10 min. Photographs were taken using a laser-scanning confocal microscope. Pictures 1–3 are fluorescent images, 4–6 are overlapped images. (C) Changes in stomatal aperture of turnip leaves treated with pst DC3000 for 0, 1, and 3 h. Stomatal aperture size (mean values ± SE, n = 90 stomata from three leaves) of leaves treated with pst DC3000 for 0, 1, and 3 h. Different letters within a column indicate a significant difference (p < 0.05; Tukey’s test).
