Figure 3 | Scientific Reports

Figure 3

From: Phosphatidylserine decarboxylase CT699, lysophospholipid acyltransferase CT775, and acyl-ACP synthase CT776 provide membrane lipid diversity to Chlamydia trachomatis

Figure 3

Sensitivity of the C.t. acyl-ACP synthase AasC to acyl-CoA synthetase inhibitors. AasC (CT776) was expressed in E. coli, and the incorporation of 5 µM 14C-C16-OH was determined in crude lysates in the presence of 5 µM triacsin C and 100 µM rosiglitazone G, as indicated. The reactions were performed at 30 °C; the rate of 14C-PL formation was calculated from measurements of 4 samples taken at intervals between 0 and 8 min. The values obtained in the absence of the drugs were used to calculate % inhibition in their presence. (A) E. coli proteins before and after induction of expression of C.t. AasC were separated by SDS-PAGE and stained with the GelCode Blue dye. Panels B and C. Quantification of the incorporation of 14C-C16-OH in the presence of triacsin C (panel B) and rosiglitazone G (panel C) is reported as percentage relative to the incorporation observed in their absence. Three independent experiments were performed and the error bars indicate the standard deviation of 3 measurements.

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