Figure 4 | Scientific Reports

Figure 4

From: Phosphatidylserine decarboxylase CT699, lysophospholipid acyltransferase CT775, and acyl-ACP synthase CT776 provide membrane lipid diversity to Chlamydia trachomatis

Figure 4

Labeling of Chlamydia-infected cells with NBD-PS. HeLa cells were grown on coverslips and infected with C. trachomatis strain D. After 24 hours, 1 µM NBD-PS (green) was added to the medium. The cells were washed and fixed after 1 hour of incubation. DNA was stained with Hoechst dye (blue), and imaging was performed with a Keyence microscope equipped with a 40x objective. Panel B shows a magnified cropped image of an infected cell with 2 inclusions, which are indicated. Images were taken from a single labeling experiment.

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