Figure 5 | Scientific Reports

Figure 5

From: Phosphatidylserine decarboxylase CT699, lysophospholipid acyltransferase CT775, and acyl-ACP synthase CT776 provide membrane lipid diversity to Chlamydia trachomatis

Figure 5

CT699 is a PS-decarboxylase enzyme. CT699 was expressed in E. coli (panel A), and activity measurements were performed in the presence of 2 µM NBD-PS at 37 °C for 20 min (panel B, lane PS + CT699) or from 0 to 6 minutes (panel C). Control reactions were performed using E. coli cells without the cloned CT699 construct (vector, panel C). Lipids were extracted and separated by thin-layer chromatography using unreacted NBD-PS and NBD-PE as migration standards (panel B and insert panel C). Several independent experiments were performed and the error bars in panel C indicate the standard deviation of 3 measurements of the samples obtained in one experiment.

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