Figure 2 | Scientific Reports

Figure 2

From: Ml proteins from Mesorhizobium loti and MucR from Brucella abortus: an AT-rich core DNA-target site and oligomerization ability

Figure 2

Mls binding to the Exo43bp, Seq1, Seq2 and Seq3 double-stranded oligonucleotides by EMSA. The nucleotide sequence of the Exo43bp is shown and the sequences of the three 20 bp long oligonucleotides, Seq1, Seq2 and Seq3, are also indicated. (a) EMSA with Ml1; (b) EMSA with Ml2; (c) EMSA with Ml258–141; (d) competition assays with Ml1 using Seq1 and Seq3 as competitors (Comp.) and the FAM-labelled Seq3 as a probe; (e) competition assays with Ml2 using Seq1 and Seq3 as competitors (Comp.) and the FAM-labelled Seq3 as a probe. The amounts of the competitors are indicated on the top of the lanes as excess with respect to the probe (from 12.5-fold to 100-fold excess). Full-length gels are presented in Supplementary Figs S8–S12.

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