Figure 4

Mls binding to the core double-stranded oligonucleotides (a–d) and competition experiments (e–l). The sequences of the oligonucelotides tested as target sites and as competitors (Comp.) are shown. The competition experiments were perfomed by adding 25-, 50- or 100-fold excess of each competitor to the reaction mixture with respect to the amount of the FAM-labelled Seq3 double-stranded oligonucleotide used as the probe. Full-length gel are presented in Supplementary Figs S17–S26.