Figure 6

Brucella MucR binding to the target site for the Mls. (a) EMSA of MucR binding to the double-stranded oligonucleotides Exoy43bp, Seq1, Seq2 and Seq3; (b) EMSA of MucR binding to the double-stranded oligonucleotides Seq1.1, Seq1.2, Seq3.1 and Seq3.2; (c) competition assay of MucR binding to double-stranded Seq3 in the presence of a 1:1 molar ratio of netropsin. Full-length gels are presented in Supplementary Figs S31–S33.