Figure 1 | Scientific Reports

Figure 1

From: Metformin transiently inhibits colorectal cancer cell proliferation as a result of either AMPK activation or increased ROS production

Figure 1

Metformin (Met) decreased the proliferation, migration, and invasion of HT29, HCT116 and HCT116 p53−/− cells. (a) Cell proliferation was evaluated in vitro by means of BrdU incorporation in the absence (Ctrl) or presence of 5 mM Met after 24, 48 and 72 hours’ treatment. The results are shown as mean values ± SD compared with the control group (**P < 0.01, ****P < 0.0001). (b) The wound healing assay was conducted after Met treatment (0.6 mM for HT29 and HCT116 p53−/−; 1.25 mM for HCT116) for 90 hours (HT29), 38 hours (HCT116) or 40 hours (HCT116 p53−/−). (c) The chamber invasion assay was performed after treatment with 0.6 mM or 1.25 mM Met for 96 hours (HT29) or 72 hours (HCT116 and HCT116 p53−/−).

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