Figure 7

Metformin (Met) affects the expression of the CRC stemness markers LGR5 and CD44. (a) qRT-PCR of CD44 and LGR5 in HT29, HCT116 and HCT116 p53−/− cells. (b) In situ hybridisation of LGR5 in HT29 cells. Hematoxylin and eosin (H&E) staining is the same as in Fig. 2.