Figure 7
TRPA1 inhibitors and activators affected GVNA in rats and β-eudesmol activated rat TRPA1. (A) Representative traces of whole-cell patch-clamp currents activated by β-eudesmol (60 μM) in HEK293 cells expressing rat TRPA1. AITC (100 μM) was used as a TRPA1 stimulant. (a), (b) Indicate the points of ramp pulse application to generate curves I–V shown in (B,C,D). The effect of a selective TRPA1 inhibitor, HC-030031 (300 mg/kg), on β-eudesmol derived GVNA elevation (n = 3). (E,F). The effect of AP-18 (1 mg/kg), a selective TRPA1 inhibitor, on β-eudesmol derived GVNA elevation (n = 3). (G,H). The effect of a non-selective TRPA1 inhibitor, Ruthenium Red (1 mg/kg), on β-eudesmol derived GVNA elevation (n = 3). (I,J). The effect of a representative TRPA1 activator, AITC, on GVNA (n = 5). Values are means ± SEM. Statistical differences were analyzed by the Mann-Whitney U test. *p < 0.05; **p < 0.01. AITC, allyl isothiocyanate; CTL, control; β-EUD, β-eudesmol; HC03, HC-030031; RR, Ruthenium Red.
