Figure 4 | Scientific Reports

Figure 4

From: Inhibition of intimal hyperplasia in murine aortic allografts by administration of a small-molecule TLR4 inhibitor TAK-242

Figure 4

TAK-242 downregulates proinflammatory cytokines in grafts and NF-κB signaling pathway in graft macrophages. TAK-242 treatment significantly reduced expression of (A) IL-12 (P = 0.0491), (B) IL-6 (P = 0.012), TNFα (P = 0.016), iNOS (P = 0.005), IL-1β (P = 0.043), and (C) CCL2 (P < 0.001) and increased expression of (A) IL-4 (P < 0.001) in allografts as compared with vehicle treatment. There was no significant difference in the expression of other genes (A,B,C,D) between the two groups. (E) TAK-242 treatment significantly decreased TRAF6 (P = 0.036) and CCL2 (P = 0.023) expression and decreased phosphorylation of IκBα (P = 0.010) and p65 (P = 0.049) in graft macrophages, as compared with vehicle treatment. (n = 4 mice for per group *P < 0.05, **P < 0.05, ***P < 0.001).

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