Figure 3
Validation of indentation data classification in a dedicated experiment with enzymatic degradation of the glycocalyx. (A) Data for the control aorta sample. (B) Data for the aorta sample after hyaluronidase incubation and partial glycocalyx degradation. Both panels show data as scatter plots of the elastic modulus at maximal load EML versus brush length L. The contour lines show Gaussian mixture distributions with two components fit to the data. The data points shown in blue (type 1 curves - without the glycocalyx) and red (type 2 curves - with the glycocalyx) were classified using an automatic clustering procedure. The histograms show the marginal distributions of EML and L (white bars – all data points, red bars – red data points). The glycocalyx degradation in (B) is manifested as an increase in the relative number of blue data points.
